Research Articles
A Comprehensive Guide to Validating Gene Expression Patterns with Multiple In Situ Hybridization Probes
This article provides a detailed framework for researchers, scientists, and drug development professionals on validating gene expression patterns using multiple in situ hybridization (ISH) probes.
Spatial Validation of Single-Cell RNA Sequencing Data: A Comprehensive Guide to ISH Techniques and Best Practices
This article provides researchers, scientists, and drug development professionals with a comprehensive framework for validating single-cell RNA sequencing (scRNA-seq) findings using in situ hybridization (ISH) techniques.
Advanced Signal Amplification Methods for Low-Abundance Targets: From Molecular Tools to Clinical Diagnostics
This comprehensive review explores cutting-edge signal amplification technologies that enable sensitive detection of low-abundance molecular targets, addressing a critical challenge in biomedical research and clinical diagnostics.
Formamide in Hybridization Buffer: A Comprehensive Guide to Mechanism, Optimization, and Safer Alternatives
This article provides a complete resource for researchers and drug development professionals on the critical role of formamide in hybridization buffers.
Optimizing Tyramide Signal Amplification FISH for Planarian Research: A Comprehensive Guide from Principles to Practice
This article provides a comprehensive resource for researchers applying Tyramide Signal Amplification FISH (TSA-FISH) in the planarian model system.
Solving Weak Signal in Whole Mount Embryo Staining: A Foundational to Advanced Troubleshooting Guide
Weak or absent signal is a pervasive challenge in whole mount embryo staining, hindering the visualization of gene and protein expression patterns crucial for developmental biology, drug discovery, and genetic...
Breaking the Barrier: Advanced Strategies for Enhanced Antibody Penetration in Thick Embryo Samples
This article provides a comprehensive guide for researchers and drug development professionals tackling the critical challenge of antibody penetration in thick embryo specimens.
Optimizing Fixation Methods for Whole-Mount Embryo Immunofluorescence: A Comprehensive Guide for Biomedical Research
Whole-mount immunofluorescence provides unparalleled three-dimensional spatial context for studying protein expression during embryonic development.
DNA vs RNA Probes for ISH: A 2025 Guide to Selection, Optimization, and Application
This article provides a comprehensive and up-to-date analysis of DNA and RNA probes for In Situ Hybridization (ISH), tailored for researchers, scientists, and drug development professionals.
Whole Mount Immunofluorescence for Mouse Embryos: A Complete Guide from Principles to 3D Analysis
This comprehensive overview details the application of whole-mount immunofluorescence (WM-IF) for the 3D spatial analysis of protein localization in mouse embryonic tissues.