Research Articles
ICE vs. CRISPR-STAT: A Comparative Analysis of Indel Detection Methods for CRISPR Genome Editing
This article provides a comprehensive comparative analysis of two prominent indel detection methods, ICE (Inference of CRISPR Edits) and CRISPR-STAT (Somatic Tissue Activity Test).
Cas9 Protein vs. mRNA Delivery: A Strategic Guide to Optimizing Editing Efficiency for Therapeutics
This article provides a comprehensive analysis for researchers and drug development professionals on the critical choice between delivering the CRISPR-Cas9 system as a pre-complexed protein (RNP) or as mRNA.
Multi-Locus Targeting with Synthetic gRNAs in Zebrafish: A Revolutionary F0 Knockout Strategy for Accelerated Biomedical Research
This article details the transformative methodology of multi-locus targeting using synthetic guide RNAs (gRNAs) in zebrafish, a technique that enables highly efficient, biallelic gene knockouts directly in the F0 generation.
Homology-Independent Knock-In in Zebrafish: A Robust Strategy for Precision Genome Editing
This article provides a comprehensive overview of homology-independent knock-in strategies for precise genome editing in zebrafish, a pivotal model in biomedical and drug discovery research.
Optimizing ssODN Repair Templates for High-Efficiency CRISPR Genome Editing
This article provides a comprehensive guide for researchers and drug development professionals on designing single-stranded oligodeoxynucleotide (ssODN) repair templates to maximize the efficiency of precise CRISPR-Cas9 genome editing via Homology-Directed...
Zebrafish in Genetic Research: A Versatile In Vivo Model for Human Disease Modeling and Drug Discovery
This article explores the established and emerging roles of zebrafish (Danio rerio) as a powerful model organism for human genetic diseases.
Zebrafish Orthologous Genes: A Powerful Platform for Human Disease Modeling and Drug Discovery
This article explores the pivotal role of zebrafish orthologous genes in modeling human diseases, a cornerstone of modern biomedical research.
Optimizing Germline Transmission in Zebrafish CRISPR Mutants: A Guide for Functional Genomics and Disease Modeling
This article provides a comprehensive overview of strategies for achieving efficient germline transmission of CRISPR-Cas9-induced mutations in zebrafish, a critical vertebrate model for functional genomics and drug discovery.
Gastruloid vs. In Vivo Embryo Transcriptome: A Comprehensive Guide for Developmental Biology and Drug Discovery
This article provides a comprehensive analysis of the transcriptional landscapes of gastruloids and in vivo embryos, serving as a critical resource for researchers and drug development professionals.
How Cell Fate Specification Modes Govern Transcriptome Evolution: From Embryogenesis to Biomedical Applications
This article synthesizes recent advances in understanding how distinct modes of cell fate specification—autonomous versus conditional—shape the evolution of transcriptomes during embryonic development.