Research Articles
Multi-Locus Targeting with Synthetic gRNAs in Zebrafish: A Revolutionary F0 Knockout Strategy for Accelerated Biomedical Research
This article details the transformative methodology of multi-locus targeting using synthetic guide RNAs (gRNAs) in zebrafish, a technique that enables highly efficient, biallelic gene knockouts directly in the F0 generation.
Homology-Independent Knock-In in Zebrafish: A Robust Strategy for Precision Genome Editing
This article provides a comprehensive overview of homology-independent knock-in strategies for precise genome editing in zebrafish, a pivotal model in biomedical and drug discovery research.
Optimizing ssODN Repair Templates for High-Efficiency CRISPR Genome Editing
This article provides a comprehensive guide for researchers and drug development professionals on designing single-stranded oligodeoxynucleotide (ssODN) repair templates to maximize the efficiency of precise CRISPR-Cas9 genome editing via Homology-Directed...
Zebrafish in Genetic Research: A Versatile In Vivo Model for Human Disease Modeling and Drug Discovery
This article explores the established and emerging roles of zebrafish (Danio rerio) as a powerful model organism for human genetic diseases.
Zebrafish Orthologous Genes: A Powerful Platform for Human Disease Modeling and Drug Discovery
This article explores the pivotal role of zebrafish orthologous genes in modeling human diseases, a cornerstone of modern biomedical research.
Optimizing Germline Transmission in Zebrafish CRISPR Mutants: A Guide for Functional Genomics and Disease Modeling
This article provides a comprehensive overview of strategies for achieving efficient germline transmission of CRISPR-Cas9-induced mutations in zebrafish, a critical vertebrate model for functional genomics and drug discovery.
Gastruloid vs. In Vivo Embryo Transcriptome: A Comprehensive Guide for Developmental Biology and Drug Discovery
This article provides a comprehensive analysis of the transcriptional landscapes of gastruloids and in vivo embryos, serving as a critical resource for researchers and drug development professionals.
How Cell Fate Specification Modes Govern Transcriptome Evolution: From Embryogenesis to Biomedical Applications
This article synthesizes recent advances in understanding how distinct modes of cell fate specification—autonomous versus conditional—shape the evolution of transcriptomes during embryonic development.
Heterochrony in Extraembryonic Development: Evolutionary Mechanisms and Biomedical Applications
This article synthesizes contemporary research on heterochrony—evolutionary changes in developmental timing—focusing on its critical role in extraembryonic development across species.
ZEB2 in Somitogenesis: Unraveling its Critical Role in Mouse and Human Development
This article synthesizes current research on the transcription factor ZEB2 and its indispensable function in mouse and human somitogenesis.