Research Articles
Optimized Photo-Bleaching of Melanophores for Enhanced Whole-Mount In Situ Hybridization in Xenopus
This article provides a comprehensive guide for researchers and drug development professionals on optimizing Whole-mount In Situ Hybridization (WISH) in Xenopus laevis by addressing the critical challenge of melanophore interference.
Tissue Permeability in Biomedical Research: Mechanisms, Assessment, and Impact on Drug Development
This article provides a comprehensive analysis of the critical role tissue permeability plays in physiological function, disease pathogenesis, and therapeutic development.
A Comprehensive Guide to Reducing Non-Specific Signal in WMISH: From Foundational Principles to Advanced Troubleshooting
This article provides a systematic guide for researchers and drug development professionals seeking to eliminate non-specific signal in Whole-Mount In Situ Hybridization (WMISH).
Crispants vs Morphants vs Mutants: A Comprehensive Guide to Phenotypic Comparison in Genetic Research
This article provides a definitive guide for researchers and drug development professionals on the critical phenotypic differences between crispants (F0 CRISPR/Cas9 mutants), morphants (morpholino knockdowns), and stable genetic mutants.
Validating Mutant Alleles: Integrating Sanger Sequencing and NGS for Robust Discovery and Diagnostics
This article provides a comprehensive guide for researchers and drug development professionals on validating mutant alleles discovered through Next-Generation Sequencing (NGS).
Boosting F0 Mutagenesis Efficiency: A Comprehensive Troubleshooting Guide for Researchers
This article provides a systematic framework for researchers and drug development professionals to diagnose and resolve low efficiency in F0 CRISPR mutagenesis.
Revolutionizing Zebrafish Research: A Comprehensive Guide to ZEG Early Selection Protocol for Enhanced CRISPR Efficiency
This article provides a comprehensive examination of the Zebrafish Embryo Genotyper (ZEG) protocol, an automated non-invasive system for rapid genotyping of live zebrafish embryos.
F0 Knockout Crispants in Zebrafish: Accelerating Behavioral Screens for Drug Discovery
This article explores the transformative potential of F0 generation knockout 'crispants' in zebrafish for high-throughput behavioral screening.
Optimizing HDR Knock-In in Zebrafish: A Comprehensive Guide from Foundations to Prime Editing
Precise genome editing via Homology-Directed Repair (HDR) in zebrafish is crucial for modeling human genetic diseases and advancing drug discovery, yet it remains challenging due to low efficiency.
Optimizing Ribonucleoprotein (RNP) Complex Microinjection in Zebrafish Embryos: A Guide for Precision Genome Editing
The direct delivery of pre-assembled Cas protein-gRNA ribonucleoprotein (RNP) complexes into zebrafish embryos via microinjection represents a transformative approach for precision genome editing.