Research Articles
Optimizing Cell Capture Efficiency in Embryo scRNA-seq: Strategies for Sensitivity, Reproducibility, and Validation
Single-cell RNA sequencing has revolutionized the study of early embryonic development, but its application to precious embryo samples is hampered by the critical challenge of cell capture efficiency.
Full-Length vs 3' End Counting scRNA-seq: A Comprehensive Guide for Embryo Research and Drug Development
This article provides a detailed comparative analysis of full-length and 3' end counting single-cell RNA sequencing (scRNA-seq) protocols, with a specific focus on applications in embryo research and preclinical drug...
Troubleshooting Poor Organoid Differentiation from Single Cells: A Comprehensive Guide for Researchers
Achieving robust differentiation in organoids derived from single cells remains a significant challenge, impacting the reliability of disease modeling and drug screening.
Optimizing Organoid Size and Shape: Engineering Strategies for Enhanced Differentiation and Function
This article provides a comprehensive overview of advanced strategies for controlling organoid size and shape to improve differentiation efficiency, functionality, and reproducibility.
A Stepwise Monolayer Protocol for Kidney Organoid Differentiation: Applications in Disease Modeling and Drug Development
This article provides a comprehensive guide to the stepwise monolayer protocol for differentiating human pluripotent stem cells into kidney organoids.
Necrosis and Hypoxic Cores in Large Organoids: Causes, Solutions, and Clinical Translation
This article provides a comprehensive analysis of the critical challenge of necrosis and hypoxic core formation in large organoids, a major bottleneck in organoid technology.
Single vs. Double Optimization Strategies in Biomedical Research: From Foundational Concepts to Advanced Applications in Drug Development
This article provides a comprehensive examination of single and double optimization frameworks and their critical applications in biomedical research and drug development.
HCR v3.0: Mastering the Low-Background Protocol for Multiplexed, Quantitative RNA and Protein Imaging
This article provides a comprehensive guide to the third-generation in situ Hybridization Chain Reaction (HCR v3.0), a revolutionary protocol that introduces automatic background suppression for superior signal-to-noise ratios.
Optimizing SDS Reduction Solutions for Whole Mount Hybridization in Spiralian Model Systems
Whole mount in situ hybridization (WMISH) is an indispensable technique for visualizing spatial gene expression, yet its application in Spiralian models presents unique challenges due to complex tissue biochemistry and...
Optimized Photo-Bleaching of Melanophores for Enhanced Whole-Mount In Situ Hybridization in Xenopus
This article provides a comprehensive guide for researchers and drug development professionals on optimizing Whole-mount In Situ Hybridization (WISH) in Xenopus laevis by addressing the critical challenge of melanophore interference.