Research Articles
Spatiotemporal Signaling in Cell Fate Decisions: From Dynamic Landscapes to Therapeutic Applications
This article synthesizes current research on how the spatial and temporal dynamics of cellular signaling govern cell fate decisions.
Spatial Multiomics: Mastering ISH and Immunohistochemistry Co-Localization for Precision Biomarker Discovery
This article provides a comprehensive guide to in situ hybridization (ISH) and immunohistochemistry (IHC) co-localization studies, a cornerstone of spatial biology.
Morphogen Patterning in Embryonic Development: Mechanisms, Models, and Biomedical Applications
This article provides a comprehensive analysis of how morphogen patterns guide embryonic development, synthesizing foundational concepts with recent methodological advances.
Nodal Signaling in Zebrafish Mesendodermal Patterning: From Molecular Mechanisms to Research Applications
This comprehensive review explores the pivotal role of Nodal signaling in zebrafish mesendoderm induction and patterning, providing researchers and drug development professionals with current mechanistic insights and practical methodologies.
ISH Probe Labeling Techniques: A Comprehensive Guide from Fundamentals to Advanced Applications
This article provides a thorough evaluation of in situ hybridization (ISH) probe labeling techniques, tailored for researchers, scientists, and drug development professionals.
Double Colorimetric In Situ Hybridization: A Comprehensive Guide to Methods, Optimization, and Validation
Double colorimetric in situ hybridization (dCISH) is a powerful technique for the simultaneous spatial localization of two distinct nucleic acid targets within cells and tissues, providing critical insights into gene...
HCR-FISH vs. CARD-FISH: A Comprehensive Sensitivity and Application Analysis for Biomedical Research
This article provides a critical evaluation of two powerful signal amplification techniques in fluorescence in situ hybridization: Hybridization Chain Reaction FISH (HCR-FISH) and Catalyzed Reporter Deposition FISH (CARD-FISH).
Preserving RNA Integrity: A Comprehensive Guide to Preventing Degradation in Your ISH Experiments
This article provides a complete framework for researchers and drug development professionals to prevent RNA degradation during in situ hybridization (ISH) protocols.
FISH vs. Next-Generation Sequencing: Assessing Concordance in Modern Clinical Diagnostics
This article provides a comprehensive analysis of the concordance between Fluorescence In Situ Hybridization (FISH) and Next-Generation Sequencing (NGS) for genomic alteration detection in clinical diagnostics.
Post-Hybridization Wash Stringency Optimization: A Complete Guide for Researchers
This article provides a comprehensive guide for researchers and drug development professionals on optimizing post-hybridization wash stringency for in situ hybridization (ISH) and fluorescence in situ hybridization (FISH) techniques.